program (as of March, 23)


Sunday, April 1
16:30-20:00Registration
18.00-18:35 Gadella Jr. T.W.J. and J. Vermeer and G.N.M. van der Krogt and R. Mirabella and R. Immink and F.J.M. van Kuppenveld GFP-based FRET microscopy in living cells
18.35-19.10 C. Cremer: Spectral Precision Distance Microscopy for the Analysis of Human Nuclear Genome Nanostructure
19:10-21:00Get Together Party
  

Monday, April 2
08:00-09:00Registration
09.00-10:45Plenary Session
 
09:00-09:35 Gu, M. Microscopic imaging through turbid tissue media: a comparison of penetration depth between single-photon, two-photon and three-photon excitation
09:35-10:10 Ludin, B. and F. del Principe Applications of fluorescent proteins in life imaging: welcome to the movies
10:10-10:45 Zumbush, A. CARS microscopy
10:45-11:15Coffee break
11:15-12:35Parallel Session
 
A Living cells I (4D-imaging)
11.15-11.35 Monier, K. and R.D. Shelby and K.F. Sulivan 4-D Imaging approaches to understanding chromosome organisation in living human cells
11.35-11.55 Manders, E.M.M. and A.E. Visser and C. Molenaar and A. Koppen and E. de Wit and W de Leeuw and R. van Liere and G.J. Brakenhoff and R. van Driel: Microscopy of living cells: How to keep cells alive
11.55-12:15 Bronnenberg, N. and H. Kuijpers and E. Manders and B. Schutte and F. Ramaekers and J. Broers Dynamics of the nuclear lamina
12:15-12:35 DeVaney, T.T.J. and H. Ahammer and H.A. Tritthart 4D imaging of invasion using Confocal Laser Scanning Microscopy and a 3D in vitro spheroid confrontation model of invasion
B Non-linear imaging I
11.15-11.35 Volkmer, A. and J. Cheng and L.D. Book and X.S. Xie: New developments on coherent anti-stokes raman scattering (CARS) microscopy: vibrational imaging and spectroscopy with high sensitivity
11.35-11.55 Müller, M. and J.M. Schins and G.J. Brakenhoff Multiplex CARS-imaging: chemical composition at microscopic resolution
11.55-12:15 Hashimoto, M. and K. Inoue and T. Araki and K. Fujita and O. Nakamura and S. Kawata Multi-focus cars microscopy
12:15-12:35 Sheppard, C.J.R. and P.B. Lukins and R. Gauderon and J.J. Bevett and P. Xu Second harmonic generation microscopy
12:35-14:00Lunch
14:00-15:20Parallel Session
 
A Living cells II (FRAP)
14:00-14:20 Jonkman, J. and N. Salmon and E.H.K. Stelzer Custom FRAP experiments on the compact confocal camera
14:20-14:40 Houtsmuller, A.B. and A.L. Nigg and M.C.E. van Poeijer and D. Hoogstraten and P. Farla and J.H.J. Hoeijmakers and W. Vermeulen Kinetics of DNA repair and gene transcription in living cells
14:40-15:00 Sporbert, A. and H. Leonhardt and M.C. Cardoso: Organization of DNA replication by transient and local immobilization of rapidly diffusing replication factors
15:00-15:20 Thomsen, P. and M. Stahlhut and B. van Deurs FRAP analysis of EGFP-caveolin-1: Caveolae are immobile plasmamembrane domains
BNon-Linear Imaging II
14:00-14:20 Potma, E.O. and W.P. de Boeij and D.A. Wiersma Mapping ultrafast motions of water in cells using nonlinear optical kerr effect microspectroscopy
14:20-14:40 Török, P. and F-J. Kao: High aperture surface second harmonic generation microscopy
14:40-15:00 Potma, E.O. and W.P. de Boeij and D.A. Wiersma Nonlinear signal accumulation in the focus of a high numerical aperture lens: spatial resolution in CARS microscopy
15:00-15:20 Cheng, P-C. and C-K. Sun and F-J. Kao and B-L. Lin: Non-linear spectral microscopy-multi-photon FL, SHG and THG
15:20-15:50Coffee break
15:50-17:10Parallel Session
 
A Living cells III (Non-Linear Imaging)
15:50-16:10 Uzunbajakava, N. and A. Lenferink and Y. Kraan and B. Willekens and G. Vrensen and J. Greve and C. Otto Raman imaging of chromatin in living cells
16:10-16:30 Canioni, L. and S. Rivet and L. Sarger and R. Barille Visualization of intracellular Ca2+ dynamic with Third Harmonic Generation microscopy
16:30-16:50 Deng, X. and M. Gu and E.D. Williams and E.W. Thompson Second-harmonic generation microscopy in human malignant prostate tissue
16:50-17:10 Sun, C-K. and S-W. Chu and I-S. Chen and T-M. Liu and F. Bresson and B-L. Lin and P-C. Cheng Multi-modality nonlinear spectral microscopy
BMicroscopy I (Instrumentation)
15:50-16:10 Benedetti, P.A. and G. Guidarini Super-confocal imaging in Video-Confocal Microscopy
16:10-16:30 Weiss, G. Recent developments in Laser Scanning Microscopy
16:30-16:50 Girkin, J. Applications of blue InGaN laser diodes to confocal imaging
16:50-17:10 Engelhardt, J. and H. Ulrich [and H. Gugel] Design considerations for a multi spectral detector for confocal microscopy and Design considerations for an optically correct fast XY-scanner arrangement with image rotation
17:10-18:15Drinks
18:15-18:55PerkinElmer-lecture by Dr. F. Bobanivic: Looking into live cells
19:00-21:00Dinner offered by PerkinElmer life sciences
  

Tuesday, April 3
09.00-10:45Plenary Session
 
09:00-09:35 Gustafsson, M.G.L. and L. Shao and D.A. Agard and J.W. Sedat Structured illumination microscopy: three-dimensional widefield imaging
09:35-10:10 L. Moreaux: Second harmonic light microscopy theory and application in membrane imaging
10:10-10:45 Heintzmann, R. and Q. Hanley and D. Arndt-Jovin and T. Jovin Imaging with 'Out-of-Focus' light: The Dual-Sided Programmable Array Microscope (PAM)
10:45-11:15Coffee break
11:15-12:35Parallel Session
 
A Living cells IV (FRET - FLIM - FCS)
11.15-11.35 Agronskaia, A.V. and H.C. Gerritsen High speed fluorescence lifetime imaging
11.35-11.55 Eils, R. and D. Gerlich and M. Gebhard and J. Fieres and J. Mattes and J. Beaudouin and J. Ellenberg 4D triple-color imaging reveals distict topology of nuclear envelope breakdown and reassembly
11.55-12:15 Hanley, Q.S. and D.J. Arndt-Jovin and T.M. Jovin Spectrally resolved fluorescence lifetime imaging microscopy (SFLIM)
12:15-12:35 Weisshart, K. Fluorescence correlation spectroscopy as a tool to probe molecular interactions inside living cells
B Microscopy II (Instrumentation)
11.15-11.35 Stelzer, E.H.K. and E.-L. Florin The Photonic Force Microscope (PFM) follows a lipids raft in the plasma membrane of mammalian cells
11.35-11.55 Kawata, S. and T. Tanaka Two-photon fabrication of micro/nano structures exceeding the diffraction limit in 3D
11.55-12:15 van Sark, W.G.J.H.M. and P.L.T.M. Frederix and D.J. van den Heuvel and A.A. Bol and J.N.J. van Lingen and T.A.H. Asselbergs and I. Senf and C. De Mello Donega and A. Meijerink and E.L. de Beer and H.C. Gerritsen Fluorescence intermittency of molecules and nanoparticles studied by time-resolved single molecule spectroscopy
12:15-12:35 Uhl, R. and J. Wietzorrek and J. Peters Novel concepts for imaging without filters
12:35-13:45Lunch
13:30-15:00
Poster session (see below for titles)
14:45-15:15Coffee and tea
15:15-16:55Parallel Session
 
A Biological Imaging I
15:15-15:35 Bolzer, A. and K. Saracoglu and C. Conrad and R. Eils and T. Cremer and M.R. Speicher Multicolor FISH in 3 dimensions
15:35-15:55 Dobrucki, J. and H.D. Halicka and Z. Darzynkiewicz Changes of in situ DNA helix stability associated with cell cycle and apoptosis
15:55-16:15 Mattern, K.A. and A.B. Houtsmuller and A.L. Nigg and J.M.J.M. Zijlmans The binding of TRF1 and TRF2 to telomeres is highly dynamic in vivo
16:15-16:35 Woldringh, C.L. and N.O.E. Vischer and S. Cunhab Image analysis of the bacterial nucleoid in intact cells and after isolation
16:35-16:55 Sharp, L. and G. Mackay and T. Pratt and D. Price and J. West Using timelapse confocal microscopy to analyse tauGFP transgene expression in preimplantation mouse embryos
B Image analysis I
15:15-15:35 Kozubek, M. and P. Matula Computer analysis of the behavior of an optical system and software correction of artifacts using fluorescent beads
15:35-15:55 Haeberlé, O. and A. Dieterlen and C. Xu and M.-P. Gramain Analysis of point spread functions from a fluorescence microscope and identification of acquisition parameters
15:55-16:15 Hanley, Q.S. and T.M. Jovin What can hadamard transforms tell you about spreading functions in a microscope?
16:15-16:35 Kriete, A. and M. Naim and L. Schäfer Quality measures in applications of image restoration
16:35-16:55 Pawley, J. B. Limits to the precision of optical sectioning in live-cell confocal microscopy
17:15Departure for excursion to Marken and Volendam
19:00Conference dinner in Volendam
22:15Return to Amsterdam Drop off at Central Station (city center) and Academic Medical Center (AMC)
  

Wednesday, April 4
09:00-10:20Parallel Session
 
A Biological Imaging II
09:00-09:20 Segers-Nolten, G.M.J. and C. Wyman and N. Wijgers and W. Vermeulen and A. Lenferink and C. Otto and J. Hoeijmakers and J. Greve Confocal fluorescence microscopy on single nucleotide excision repair complexes
09:20-09:40 Stekelenburg, M. and M.C.M. Rutten and L.H.E.H. Snoeckx Visualization of living cardiac myoblasts with AFM and CLSM
09:40-10:00 Wiegand, U.K. and A. Don-Wauchope and R.R. Duncan and J. Greaves and M.J. Shipston and D.K. Apps and R.H. Chow Reconstitution of single-vesicle exocytosis using TIRF microscopy
10:00-10:20 Schneckenburger, H. and M. Lyttek and K. Stock and W.S.L. Strauss and R. Sailer Total internal reflection fluorescence lifetime imaging (TIR-flim) of rhodamine 123 in single cells
B Image analysis II
09:00-09:20 Landmann, L. Deconvolution improves colocalization analysis of confocal 3D data sets
09:20-09:40 Adler, J. and S.N. Pagakis The importance of voxel size for the deconvolution of confocal 3D images of blood vessels
09:40-10:00 Colicchio, B. and C. Xu and O. Haeberlé and A. Dieterlen and G. Jung and E. Ginglinger and E. Jeandidier Regularization of fluorescence image deconvolution by adaptive pre-filtering
10:00-10:20 Van der Voort, H. and E.M.M. Manders Restoration in scanning disk confocal microscopy (SDCM), confocal/wide-field aspects
10:20-10:55Coffee break
10:55-12:15Parallel Session
 
A Living cells V
10:55-11.15 Oldenbourg, R. Dynamic views of living cell fine strcuture revealed by birefringence imaging
11.15-11.35 Fujita, K. and T. Kaneko and N. Smith and K. Kato and O. Nakamura and T. Takamatsu and S. Kawata Multiphoton laser stimulation of living cells
11.35-11:55 Diaspro, A., O. Cavalleri and A. Gliozzi and M. Robello and D. Silvano and G. Sukhorukov Two-photon and confocal microscopy of polyelectrolyte hollow capsules: characterization and applications
11:55-12:15 Neu, T.R. and J.R. Lawrence Evaluation of fluorchromes for 2-photon laser scanning microscopy (2-PLSM) of environmental biofilm systems
B Microscopy III (Wave propagation)
10:55-11.15 Smalyukh, I.I. and S.V. Shiyanovskii and O.D. Lavrentovich Fluorescence confocal polarizing microscopy of director structures in chiral liquid crystals
11.15-11.35 Dubois, A. and L. Vabre and A.C. Boccara White-light full-field optical coherence tomography
11.35-11:55 Booth, M.J., M.A.A. Neil, R. Juskaitis and T. Wilson Adaptive optics for confocal and multi-photon microscopy
11:55-12:15 Sheppard, C.J.R. Localization of high-aperture wave fields
12:15-13:40Lunch
13:40-15:00Parallel Session
 
A Living cells VI
13:40-14:00 Molenaar, C. and S. Khazen and K. Bergsma and E.M.M. Manders and R. Dirks and T. Raap and H. Tanke Strategies and use of FISH applied to living cells
14:00-14:20 Bush, P.G. and A.C. Hall 4D-Imaging of in situ articular chondrocyte volume regulation by confocal microscopy
14:20-14:40 Schermelleh, L. and T. Cremer Dynamics of two-color labeled, early and mid-to-late replicating chromatin studied in living cells
14:40-15:00 Stuurman, N. An open source solution for archiving of digital images: the scientific image database
B Microscopy IV
13:40-14:00 Cogswell, C.J. Video rate extended depth of focus microscopy for fluorescent, brightfield and pahse imaging
14:00-14:20 Juskaitis, R. and M.A.A. Neil and F. Massoumian and T. Wilson Strategies for wide-field extended focus microscopy
14:20-14:40 Kawata, Y. and M. Murakami and T. Tsuboi and S. Terakawa and C. Egami and O. Sugihara and N. Okamoto and M. Tsuchimori and O. Watanabe Observation of PC12 with non-optically probing near-field miscroscopy
14:40-15:00 Wilson, T. and M.A.A. Neil and R. Juskaitis and D. Karadaglic and S.E.D. Webb and J. Siegel and M.J. Cole and P.M.W. French Applications of structured light microscopy
15:00-15:35 Hell, S.W. and T.A. Klar and J. Jakobs and M. Dyba and H. Gugel Sharpening up the confocal microscope
15:35-15:45 G.J. Brakenhoff: closing remarks
15:45-16:30Farewell drinks
  

Poster presentations
   
 
Aigouy, L., De Wilde, Y., Ducourtieux, S., Fragola, A., Grésillon, S. Rivoal, J.-C., Boccara, C., Linear and non-linear near field microscopy: enhancement effect

Aksenov, Y.Y., Reinders, E.G.J., Greve, J., Van Blitterswijk, C., De Bruijn, J., Van Apeldorn, A., Otto, C. Confocal raman microspectrometer (CRM) for combination with electron microscope

Bergsma, C.B.J., Streekstra, G.J., Smeulders, A.W.M. Robust spot tracking with spot fusion and spot splitting in 3D image sequences

Bernas, T., Dobrucki, J., Subcellular localisation of MTT tetrazolium salt reduction by live cells in culture, using backscattered light confocal miscroscopy

Caprioli, M., Fascio, U., Melone, G., Ricci, C., Santo, N. Morphology of bdelloid rotifers under scanning electron and confocal laser microscopes

Duncan, R.R., Greaves, J., Matskevitch, J., Apps, D.K., Shipston, M.J., Chow, R.H. In vivo analysis of single vesicle dynamics and exocytosis

Engel, U., Holstein, T.W. 3-dimensional reconstruction of protein sorting in hydra stinging cells

Esterman, M.A., Tschöp, M., Fouts, R.L. Localization and analysis of Ghrelin Receptor (GHS-R) binding in rat brain by confocal microscopy

Floren, W., Voeten, J., Janssens, B., Molenberghs, K., Moelans, P., Van Ginckel, R., Wouters, W., Smets, G. GFP-Technology for fast assessment of in vivo tumor growth

Grill, S.W., Gönczy, P., Stelzer, E.H.K., Hyman, A.A. Polarity controls forces governing asymmetric spindle positioning in the C. elegans embryo

Hershfinkel, M., Segal, D., Sekler, I., Moran, A. Cellular Zn2+ regulation by ZnT-1 and a novel Na+/Zn2+ Exchanger

Huisken, J., Stelzer, E.H.K. Levitation and rotation of absorbing micron-sized particles

Inami, W., Kawata, Y., Radiation force determined by the multiscattering between a spherical particle and a substrate

Jacobsen, W.J. Confocal Raman Microscopy: a powerful tool for solving material science problems

Jonás, A., Zemánek, P., Jákl, P., Florin, E.-L. Influence of weak surface reflection on single beam optical trapping

Kao, F.-J., Wang, Y.-M., Chen, J.-C., Cheng, P.-C., Chen, R.-W., Lin, B.-L. Response of chloroplasts in protoplasts from Arabidopsis thaliana under single photon and multi-photon excitation

Karadaglic, D., Wilson, T. Image formation in microscopy using structured illumination

King, R.G., Murr, E.R., Carrasco, J., Anikijenko, P., Chen, Q., Delaney, P.M., Thomas, S.G., Vo, L.T. Use of confocal microscopy and fluorescent antibodies for IN VIVO detection of small subsurface human melanomas in mice

Kolcz, J., Rajwa, B., Drukala, J., Dobrucki, J., Korohoda, W., Malec, E. Three-dimensional visualisation of connexin 43 on the surface of human cardiomyocytes

Kubitscheck, U., Rohleder, D., Schmalz, D., Kues, T., Peters, R. Single molecules detected at the nuclear pore complex

Kues, T., Dickmanns, A., Peters, R., Lührmann, R., Kubitscheck, U. Single U1 snRP particles observed in cell nuclei

Mahaut-Smith, M.P., Thomas, D., Usher-Smith, J.A., Skepper, J.N., Mason, M.J. Confocal fluorescence and electrophysiological studies of the demarcation membrane system in living rat megakaryocytes

Martínez-Corral, M., Caballero, M.T., Muñoz-Escrivá, L. Reduction of axial sidelobes in 4Pi confocal microscopy by annular binary filters

Massoumian, F., Juskaitis, R., Neil, M.A.A., Wilson, T. Quantitative polarised light microscopy

Moné, M.J., Volker, M., Mulenders, L.H.F., Van Zeeland, A.A., Bernas, T., Houtsmuller, A.B., Vermeulen, W., Manders, E.M.M., Van Driel, R. Local UV-irradiation of cell nuclei results in local transcription inhibition and recruitment of repair proteins

Nevian, T., Sakmann, B. Combination of two-photon excitation fluorescence microscopy wit IR scanning Dodt gradient contrast for imaging neurons in brain slices

Nielsen, T., Fricke, M., Rüther, S., Andresen, P. Multifocal multiphoton miscrosopy for fast two-dimensional imaging without scanning

Ota, T., Sugiura, T., Kawata, S. Surface-force imaging using a laser-trapped probe

Rohrbach, A., Stelzer, E.H.K. Exploring the inside of the focal volume with trapped particles

Rooms, F., Philips, W. Estimating the blur in confocal images

Roos, M., Van Geel, A., Aarsman, M., Woldringh, C.L., Nanninga, N. Understanding DNA organisation in growing Escherichia coli by FISH in vitro and in silico

Salmon, N., Stelzer, E.H.K. The compact confocal camera: a higher-level software system built around databases

Schaefer, L.H., Schuster, D., Herz, H. Accelerated maximum likelihood based image restoration applied to three-dimensional fluorescence microscopy: a generalized approach

Schins, J., Schrama, T., Brakenhoff, G.J., Müller, M. THG Microscopy: material characterisation and image formation properties

Smalyukh, I.I., Shiyanovskii, S.V., Lavrentovich, O.D. Fluorescence confocal polarizing microscopy of director structures in chiral liquid crystals

Stanciu, G.A., Stanciu, S.G., Botez, D. CW power laser diodes investigated bij Laser Scanning Microscopy

Strack, S., Thies, W.-G., Krug, H.F. Spatial and temporal resolution of toxic effects due to TBT exposure on the cytoskeleton of living epithelial cells expressing GFP tagged F-actin

Talmor-Cohen, A., Eliyhu, E., Shalgi, R. Confocal microscopy as a tool for studing mammalian fertilization

Tischer, C., Altmann, S., Fisinger, S., Florin, E.-L. Three-dimensional thermal noise imaging

Tuinhof, R., Kolk, S.M., Roubos, E.W., Celio, M.R., Ibrahim, M. Hypothalamic innervation of rat and frog adenohypophysis

Van Bruaene, N., Joss, G., Braeckmans, K., Demeester, J., De Smedt, S., Van Oostveldt, P. Focus on the migrating nucleus in living root hairs of Arabidopsis thaliana

Van der Laan, A., Molenaar, C., Verwoerd, N., Dirks, R., Raap, T., Tanke, H. Detection of RNA in situ using FRET probes

Van Rooij, G.J., Zwier, J.M., Hofstraat, J.W., Brakenhoff, G.J. Photobleaching kinetics used for calibration of illumination intensity distributions in fluorescence microscopy

Vellinga, W.P., Rastogi, R., Vd. Bosch, M., Meijer, H.E.H. In-situ microscopy of deformation mechanisms in polymer-metal laminates

Verschure, P.J., Van der Kraan, I., Enserink, J. Moné, M., Manders, E.M.M., Van Driel, R. Functional organization of the cell nucleus: a key to eukaryotic gene control

Wessel, S., Pagel, S., Lucas, F., Hintze, U., Wepf, R. Dynamic distribution of emulsions on skin visualised by confocal laser scanning microscopy

Windoffer, R., Strnad, P., Leube, R.E. Live cell image system detecting cell cycle- and phosphorylation-dependent reorganisation of the intermediate filament cytoskeleton

Zemánek, P., Jonás, A., Jezek, J., Serý, M., Liska, M. Parametric study of single beam and standing wave optical traps